Protein phosphorylation is a ubiquitous and key step in signaling pathways. For flow cytometry staining, the right protocol can help accurately capture these phosphorylated targets for clear-cut data.

Successful flow cytometry experiments start long before your samples reach the analyzer. Every step matters—from tissue isolation and cell stimulation to staining and viability checks, each ...

In this case study, researchers at the Weizmann Institute of Science in Israel developed and optimized an imaging flow cytometry (IFC) protocol to provide a high-throughput, quantitative and highly ...

ANTIBODY TITRATION is critical to the success of any flow cytometry experiment. This is even more so in microflow cytometric analysis of extracellular vesicles (EVs), where removing excess antibodies ...

insights from industryNatalie Strickland Global Head of Translational ScienceSynexa Life Sciences In this interview, News Medical speaks with Natalie Strickland, Global Head of Translational Science ...

If you work in cell biology or immunology, chances are that you're familiar with flow cytometry. This technique measures and analyzes biological cells by suspending them in a stream of fluid and ...

Human umbilical vein endothelial cells (HUVECs) were seeded at 1 × 10 4 cells per cm 2 in six-well plates. Treatment with TNF-α (2.5, 5 or 10 ng/ml) or lipopolysaccharide (0.5, 1 or 5 μg/ml) treatment ...

Flow cytometry is a way to look closely at the features of cells or particles. A sample of blood or tissue goes into a machine called a cytometer. In less than a minute, a computer can analyze ...

Chinese clinicians used multiparameter flow cytometry on fresh bone lesion tissue to confirm Langerhans cell histiocytosis in two children, showing the technique can provide rapid ancillary diagnosis ...